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Brucella abortus pre-tmRNA (precursor permuted):

    .   10    .   20    .   30    .   40    .   50
GUCGGCGCAAGCCGACUAUGGUGAUAAACGGACGGUGUAAUAAACCCAUU
GGACCCGGGGGCGGUACCCGGCGCCUCCACCAcagcacagcuugccgauc
aggcuguguuguggcGGGGGCGAAACAGGAUCGACAAGGGUGUAAAGAUC
GCUCUUUUACUCGGCAUGAUACCACCGUUAUCGGGUCAACAGAGUAGUUG
CAAAUGACAACAAUGCUCAGGGUUAUGCUCUCGCUGCCUAAUGGCGGUGC
GGGAAACCCACUCUAAAGUCCUUAGGGUUAGCCCCUUAAGGCGGGGUUCG
GAGGCACCUGGCAACAGAAGCCUCCACUU
Two-Piece Mature tmRNA: Acceptor piece, nt 1-79; Coding piece, nt 116-329;
Internal Spacer, nt 80-115
{NOTE: Identical to Brucella suis tmRNA and mature Brucella melitensis tmRNA}
Proteolysis Tag: (A)ANDNNAQGYALAA*
Reading Frame (underlined): nt 200-241
Taxonomy: Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Brucellaceae
Sequencing:
Brucella abortus biovar 1 str. 9-941, nt 1372529-1372857 of AE017223.1 (and nt 1372529-1372857 of NC_006932.1) [Ref 1];
Brucella melitensis biovar Abortus, nt 1369679-1370007 of AM040264.1 (and nt 1369679-1370007 of NC_007618.1) [Ref 2]
Identification: [Ref 3] in sequence data at University of Minnesota Microbial Genome Program
Reference:
1. Halling et al (2005) J Bacteriol 187:2715-26
2. Chain et al (2005) Infect Immun 73:8353-61
3. K Williams (30-Jan-2002)

Posted: 30-Jan-2002
tmRNA Website ID: Bruce_abort_0221
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