For more OMX information and resources.

Offering resolution at twice the diffraction limit of traditional microscopes, the DeltaVision|OMX system is the practical solution for three dimensional super resolution imaging that does not depend on special fluorophores or fluorescent proteins. The OMX is also set-up for fast conventional imaging of live cells.

Features:

• Structured Illumination mode for high-resolution imaging. X,Y resolution to ~ 100nm, Z resolution to ~ 300nm.
• Conventional Illumination mode for fast live cell imaging. Up to 65 frames/second in four colors.
• Lasers lines (405, 488, 561, 642) and and emission filters (419-465, 500-550, 609-654, 665-705) for imaging DAPI, green, red and far red fluorophores.
• Four Photometrics Cascade II EMCCD cameras allow simultaneous four color imaging.
• Environmental control for live imaging.
• Controlled by DV-OMX software, image processing by SoftWorx imaging software.

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Purchased September 2008, the 855 is a versatile system for high-content confocal imaging of live or fixed samples. It can handle specimen formats from single slides to multiwell plates. It can automatically image multiple sites per slide or well and every well of a multiwall plate. The 855 includes powerful imaging software to perform a broad range of fluorescence-based kinetic and endpoint biological assays.

Features:

• Confocal fluorescence, widefield fluorescence, brightfield.
• X-cite lamps to provide full spectrum illumination (340nm-700nm).
• Hamamatsu Orca ER cooled CCD camera. - Handles slides to multiwell plates.
• Laser and image based autofocus.
• Environmental control. - Liquid handling.
• Robotics to handle loading/changing of multiwell plates.
• BD Pathway imaging software and BD AttoVision Software for image processing and analysis.

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Also acquired September 2008, the 435 is similar to the 855, but specialized for imaging fixed specimens. It can handle specimen formats from single slides to multiwell plates. It can automatically image multiple sites per slide or well and every well of a multiwall plate. Both machines include the same powerful imaging software to perform a broad range of fluorescence-based kinetic and endpoint biological assays.

Features:

• Confocal fluorescence, widefield fluorescence, brightfield.
• X-cite lamp to provide full spectrum illumination (340nm-700nm).
• Hamamatsu Orca ER cooled CCD camera.
• Handles slides to multiwell plates.
• Laser and image based autofocus.
• BD Pathway imaging software and BD AttoVision Software for image processing and analysis.

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For more SP5 information and resources.

Installed June 2008, the SP5 is a scanning confocal with all of the bells and whistles.  This system has 4 sensitive detectors enabling the simultaneous imaging of up to 4 different wavelengths.  It has 3 lasers providing 7 excitation wavelengths and a prism spectrometer to control emission wavelengths to make this a very versatile system compatible with most combinations of fluorophores.

Features:

• Leica DMI 6000 CS inverted microscope platform
• 7 lines of laser illumination (458,476,488,496,514,561 and 633nm) can excite from CFP to CY-5 fluorophores.
• Can simultaneously image fluorescence and brightfield or DIC
• FRAP or FRET fully supported with rapid kinetic analysis
• Controlled by Leica LAS software

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This system is optimized for live-cell imaging.  The sensitive Cascade-II EMCCD camera in conjunction with the Yokogawa CSU-10 spinning disk confocal head enables images to be captured under low light conditions with excellent temporal resolution.  The reduced photobleacing/toxicity of this system allows imaging over longer time periods than our other systems.  

Features:

• Nikon TE2000U Inverted Microscope platform
• FRAP/Photoactivation using the Mosaic System (Photonics Inc.)
• Lasers for Green or Red fluorophores (ie. GFP. FITC, RFP, TRITC)
• Can also collect bright-field and DIC images Temperature control available
• Controlled by Metamorph imaging software

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Installed May 2012, this versitile system is built on the Nikon Eclipse Ti inverted microscope. It includes the A1 scanning confocal with four lasers that provide five excitation wavelengths, four sensitive fluorescence detectors and can image in both standard or spectral mode.  This microscope includes the Perfect Focus system to maintain focus throughout long-term live imaging experiments. Also included are a very nice camera and a TIRF module for imaging high resolution fluorescence in regions very near the coverslip.

Features:

• Nikon Eclipse Ti inverted microscope platform.
• The Nikon Perfect Focus system
• Five laser lines(447, 488, 514, 561, and 640nm) to excite many of the popular fluorophores from CFP to Cy5.
• Simultaneous or sequential multichannel imaging.
• Standard filter based imaging or Spectral detector for added flexabilty in the separation of fluorophores.
• TIRF module with a 100X TIRF objective.
• Hammamatsu Orca-Flash 4.0 sCMOS camera.
• Controlled by Nikon Elements software.

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The Applied Precision DeltaVision personalDV Live Cell Imaging System is optimized for deconvolution microscopy. Deconvolution is a software technique that mathematically de-blurs images.  This system features a very nice motorized stage for precise control of X, Y, and Z plane movements.  Multiple sites on a slide can be marked and revisited.

Features:

• Olympus IX-71 Customized Inverted Microscope Stand
• Photometrics CoolSNAP HQ2 CCD camera
• Insight Solid State Illumination system with filters for DAPI, CFP, GFP/FITC, YFP, RD-TR-PE, mCherry/Alexa594, Cy5 fluorophores.
• Can also collect bright-field and DIC images
• WeatherStation Environmental Chamber to control temperature and carbon dioxide levels for live cell imaging
• Controlled by SoftWorx imaging software

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The Veritas is a fully automated microscope system designed to dissect specific cells, organelles, or tissues from your specimen for further analysis.  It can do both laser-cutting for a large number of cells and laser capture for single cells or organelles.

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There are two image processing workstations:

1) A 64 bit Image Processing workstation with a 30" high-resolution screen. Featured software includes:

• Imaris for 3-D image reconstruction, modeling analysis and presentation.
• AutoQuant for image deconvolution.

2) A 32 bit workstation with offline versions of most of our imaging software including:

• MetaMorph
• Leica Aquisition Software
• EZ-C1
• BD Attovision
• Image J

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