Indiana University Bloomington
Light Microscopy Imaging Center

News and Announcements
  • TIRF (Total internal reflection fluorescence) microscopy is now available in the LMIC. MetaCyt funds were used to add a TIRF module, a 100X TIRF objective and a very nice Orca-Flash 4.0 sCMOS camera to the A1 confocal. For more information or training, email Jim at LMIC. (May, 2012).
  • We are improving the LMIC GPA! We have replaced the Nikon C1 confocal with Nikon's top of the line A1 confocal. The A1, purchased with MetaCyt funds, is a scanning confocal with five laser lines (447, 488, 514, 561, and 640nm), multiple pmt's for simultaneous multichannel imaging and a spectral detector running on Nikon Elements software. It is a really nice microscope. For more information or training, email Jim at LMIC. (May, 2012).
  • One of Nikon's newest microscopes, the Eclipse NiE advanced research microscope, has been installed to replace the E800 widefield microscope. The NiE was purchased with LMIC funds. It features a high resolution Orca-Flash 2.8 sCMOS camera, a motorized focus stage and Nikon Elements software. For more information or training, email Jim at LMIC. (May, 2012)
  • The Applied Precision OMX Super Resolution imaging system (http://www.api.com/superres/DVOMX.asp), purchased with an NIH shared instrumentation grant (ARRA funds) is installed and ready for use. This system uses structured 3D-illumination to achieve twice the resolution possible on any of our other light microscopes in the X, Y and Z planes. Also included is a module for fast live cell imaging and a complete personalDV DeltaVision system (July 2010).
  • A 64 bit Image Processing workstation is available for use. Featured software includes AutoQuant for image deconvolution and Imaris for 3-D image reconstruction, modeling and presentation. For more information or training, email Jim at LMIC. (January 2010)
  • Both the BD Pathway 855 and 435 high-throughput confocal screening microscopes, purchased with an NIH instrumentation grant, are installed and ready for use.  These microscopes can significantly increase your imaging output. They can handle formats from slides to 384 well plates and beyond. The 855 is equipped for live cell imaging. Start imagining what these machines can do for your research. For more information contact LMIC or see the BD brochure by downloading it here: http://www.bdbiosciences.com/bioimaging/cell_biology/pathway/BDPathwayBrochure.pdf (January 2009)
Calendar: LMIS=red, DeltaVision=teal, E800 widefield=yellow, Leica confocal=brown, spinning disk=greenScheduling/Reservations

Imaging Center equipment availability can be viewed on the calendar below. Registered users may reserve microscope time by clicking on the "Google Calendar" button at the bottom right corner of the calendar (please include phone # or e-mail). To register with the imaging center or to make a training appointment, e-mail LMIC or call 812-856-1734.

You may reserve time up to two weeks in advance. Please be considerate of the needs of others by reserving only 4 hrs/day between 9am and 5pm. Remember to release your time if your plans change (Charges may apply – see policy page).